A Trimethylguanosine Synthase1-like (TGS1) homologue is implicated in vernalisation and flowering time control.

Publication Overview
TitleA Trimethylguanosine Synthase1-like (TGS1) homologue is implicated in vernalisation and flowering time control.
AuthorsTaylor CM, Garg G, Berger JD, Ribalta FM, Croser JS, Singh KB, Cowling WA, Kamphuis LG, Nelson MN
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2021
CitationTaylor CM, Garg G, Berger JD, Ribalta FM, Croser JS, Singh KB, Cowling WA, Kamphuis LG, Nelson MN. A Trimethylguanosine Synthase1-like (TGS1) homologue is implicated in vernalisation and flowering time control.. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2021 Jul 13.

Abstract

KEY MESSAGE
A plant-specific Trimethylguanosine Synthase1-like homologue was identified as a candidate gene for the efl mutation in narrow-leafed lupin, which alters phenology by reducing vernalisation requirement. The vernalisation pathway is a key component of flowering time control in plants from temperate regions but is not well understood in the legume family. Here we examined vernalisation control in the temperate grain legume species, narrow-leafed lupin (Lupinus angustifolius L.), and discovered a candidate gene for an ethylene imine mutation (efl). The efl mutation changes phenology from late to mid-season flowering and additionally causes transformation from obligate to facultative vernalisation requirement. The efl locus was mapped to pseudochromosome NLL-10 in a recombinant inbred line (RIL) mapping population developed by accelerated single seed descent. Candidate genes were identified in the reference genome, and a diverse panel of narrow-leafed lupins was screened to validate mutations specific to accessions with efl. A non-synonymous SNP mutation within an S-adenosyl-L-methionine-dependent methyltransferase protein domain of a Trimethylguanosine Synthase1-like (TGS1) orthologue was identified as the candidate mutation giving rise to efl. This mutation caused substitution of an amino acid within an established motif at a position that is otherwise highly conserved in several plant families and was perfectly correlated with the efl phenotype in F2 and F6 genetic population and a panel of diverse accessions, including the original efl mutant. Expression of the TGS1 homologue did not differ between wild-type and efl genotypes, supporting altered functional activity of the gene product. This is the first time a TGS1 orthologue has been associated with vernalisation response and flowering time control in any plant species.

Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
ChittickChittickcultivar
GeebungGeebungcultivar
Chittick/Geebung-F6Chittick/Geebung-F6population
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Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Lupin-Flowering_time-Taylor-2021
Featuremaps
This publication contains information about 1 maps:
Map Name
Lupin-Chittick/Geebung-F6
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2021 Jul 13
Journal AbbreviationTheor Appl Genet
DOI10.1007/s00122-021-03910-2
Elocation10.1007/s00122-021-03910-2
Copyright© 2021. The Author(s).
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryGermany
Cross References
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DatabaseAccession
PMID: PubMedPMID:34258645